Principles and Clinical Applications of Flow Cytometry, 4th Edition
Self-Study 060.3 – Intermediate to Advanced Level
Contact Hours (Points): 13
Written by Tsieh Sun MD in 2003.
Revised in 2014 by Susan A. McQuiston, JD, MLS(ASCP).
Online – 060.3: Course Taken Online: $126.00 BUY NOW
IMPORTANT: This online course is accessible using the following:
Desktop or Laptop Computer – Download the latest Adobe Reader program.
Kindle Fire – No additional App download is necessary.
Apple iPad (all versions) / Nook / Kindle – Recommend purchase of a PDF Reader App, such as GoodReader, Cloudreaders, or Stanza by Amazon. Once downloaded, launch this App to access the course online site in order to logon to and view your course.
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“Excellent course to continue learning about flow cytometry from the basics.”
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“The course exam was presented very clear and concise. It was excellent.“
“This course has increased my knowledge and understanding of flow cytometry. Our hematology lab has a flow cytometry dept and now I know what goes on in there when we drop off the samples for testing.“
“A great course but a lot of detailed information. I really learned a lot about flow cytometry
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TABLE OF CONTENTS
SECTION 1: INTRODUCTION
SECTION 2: INSTRUMENTATION
Fluid Transport System; Optical System; Fluorescent Signals; Lens, Filter, and Mirror; Electronic System; Computer System; Scattergram (dot plot, cytogram); Single Histogram; Contourgram (contour plot, contour map, contour histogram); Isometric Plot (three-dimensional isometric curve); Cell Sorter
SECTION 3: QUALITY ASSURANCE
Factors Affecting Flow Cytometry Results; Specimen Problems; Reagent Problems; Cell Size; Cytoplasmic Granularity; Immunophenotyping; Comparison of Percentages of Reactive Cells; The Intensity of Immunofluorescence of Surface Immunoglobulin; Double-labeling; DNA and RNA Contents; Cells That Are Not Distinguishable; Designing an 8-10 Color Assay; Current FDA Requirements Related to Flow Cytometry Antibody Conjugate Cocktails
SECTION 4: CLINICAL APPLICATION
Immunophenotyping; Developmental Stages of Hematopoietic Cells; Classification of Hematologic Neoplasms; Classification of Acute Leukemia; Classification of Chronic Leukemia; Classification of Lymphoma; Monoclonal Antibodies; Lineage-associated Antigens; Activation Antigens; Histocompatibility Antigens; Adhesion Molecules; Proliferation-associated Antigens; Gating Strategy; Criteria for Flow Cytometric Diagnosis of Hematologic Neoplasms; Immunoglobulin Light-Chain Restriction; Loss of Surface Immunoglobulin in a B-Cell Population; Coexistence of Two Different Cell-Lineage Markers on the Same Cell Population; Expression of Immature Cell Markers in a Large Number of Cells; Selective Loss of One or More Cell-Lineage Antigens; Analysis of T-cell Receptor-Vâ (TCR-Vâ) Repertoire; Selection of Monoclonal Antibody Panels; Standard Panel; Two-Tiered Approach; Targeted Approach; Immunophenotyping of Acute Leukemia; Immunophenotyping of Lymphoma and Chronic Lymphoid Leukemia; Chronic Lymphocytic Leukemia/Small Lymphocytic Lymphoma; Mantel Cell Lymphoma; Prolymphocytic Leukemia; Follicular Lymphoma; Burkitt Lymphoma (BL) and Diffuse Large B-cell Lymphoma (DLBCL); Hairy Cell Leukemia; Marginal Zone B-Cell Lymphoma; Natural Killer Cell/Natural Killer-Like T-Cell Lymphoma; Peripheral T-Cell Lymphoma; Immunophenotyping of Plasmaproliferative Disorders; Detection of Minimal Residual Disease (MRD); Flow Cytometry Panels; Introduction; Chronic Lymphocytic Leukemia/Small Lymphocytic Lymphoma (CLL/SLL); Mantle Cell Lymphoma (MCL); Lymphoma of Follicular Cell Origin (FL); Hairy Cell Leukemia (HL); Plasma Cell Myeloma (PCM); Peripheral T-cell Lymphoma; Gamma-delta T-cell Lymphoma (G-D TCL); Mycosis Fungoides / Sezary Syndrome (MF/SS); Extranodal NK/T-cell Lymphoma, nasal type (NK/T L); Angioimmunoblastic T-cell Lymphoma (AITCL); Acute Lymphoblastic Leukemia (ALL); Acute Myeloid Leukemia (AML); Hematogones; Supplementary Techniques to Immunophenotyping; Cytochemical Staining; Immunohistochemistry; Molecular Biology; Cytogenetics; Lymphocyte Subset Analysis for Immunodeficiency States; Clinical Application; Technical Consideration; DNA Ploidy Analysis; The Cell Cycle; Ploidy; Major Parameters in DNA Analysis; DNA Index (DI); Coefficient of Variation (CV); G2M Peak versus Tetraploid Peak; Doublet Discrimination; S-Phase Fraction.; Clinical Application of DNA Analysis in Hematologic and Nonhematologic Neoplasms; Aneuploidy; S-phase Fraction; RNA Index; Reticulocyte Analysis; Clinical Application; Technical Consideration
SECTION 5: CASE STUDIES
Case 1; Case 2; Case 3; Case 4; Case 5; Case 6; Case 7; Case 8; Case 9; Case 10; Case 11; Case 12; Case 13; Case 14; Case 15; Case 16; Case 17; Case 18; Case 19; Case 20