CACMLE Hematology Courses

060.3-Online: Principles and Clinical Applications of Flow Cytometry, 4th Edition


Contact Hours (Points): 13

This revised course includes two major parts. The first part concerns technical principles, including the structure and mechanism of instrumentation and quality control. The second part discusses clinical applications of flow cytometry, including immunophenotyping of hematologic neoplasms, lymphocyte subset analysis for immunodeficiency states, DNA analysis for hematologic neoplasms and solid tumors, and reticulocyte counting. After completing the course, participants should be able to understand how the instrument works, how to select a monoclonal antibody panel, and how to interpret the data generated by flow cytometry.This course was revised in 2014.

Buy This Course At Our Store Site

Product Description

Principles and Clinical Applications of Flow Cytometry, 4th Edition

Self-Study 060.3 – Intermediate to Advanced Level

Contact Hours (Points): 13

Written by Tsieh Sun MD in 2003.

Revised in 2014 by Susan A. McQuiston, JD, MLS(ASCP).

Online – 060.3: Course Taken Online: $126.00 BUY NOW

IMPORTANT: This online course is accessible using the following:

Desktop or Laptop Computer – Download the latest Adobe Reader program.

Kindle Fire – No additional App download is necessary.

Apple iPad (all versions) / Nook / Kindle – Recommend purchase of a PDF Reader App, such as GoodReader, Cloudreaders, or Stanza by Amazon. Once downloaded, launch this App to access the course online site in order to logon to and view your course.

Here’s what satisfied customers have to say~

“Excellent course to continue learning about flow cytometry from the basics.”
~Erica R.

“A very deep venture in to the subject. Not for beginners.“
~Richard D.

“The course exam was presented very clear and concise. It was excellent.“
~Hasmin C.

“This course has increased my knowledge and understanding of flow cytometry. Our hematology lab has a flow cytometry dept and now I know what goes on in there when we drop off the samples for testing.“
~Carolyn A.

“A great course but a lot of detailed information. I really learned a lot about flow cytometry
and related diseases that I did not know.“
~Tama G.




Fluid Transport System; Optical System; Fluorescent Signals; Lens, Filter, and Mirror; Electronic System; Computer System; Scattergram (dot plot, cytogram); Single Histogram; Contourgram (contour plot, contour map, contour histogram); Isometric Plot (three-dimensional isometric curve); Cell Sorter


Factors Affecting Flow Cytometry Results; Specimen Problems; Reagent Problems; Cell Size; Cytoplasmic Granularity; Immunophenotyping; Comparison of Percentages of Reactive Cells; The Intensity of Immunofluorescence of Surface Immunoglobulin; Double-labeling; DNA and RNA Contents; Cells That Are Not Distinguishable; Designing an 8-10 Color Assay; Current FDA Requirements Related to Flow Cytometry Antibody Conjugate Cocktails


Immunophenotyping; Developmental Stages of Hematopoietic Cells; Classification of Hematologic Neoplasms; Classification of Acute Leukemia; Classification of Chronic Leukemia; Classification of Lymphoma; Monoclonal Antibodies; Lineage-associated Antigens; Activation Antigens; Histocompatibility Antigens; Adhesion Molecules; Proliferation-associated Antigens; Gating Strategy; Criteria for Flow Cytometric Diagnosis of Hematologic Neoplasms; Immunoglobulin Light-Chain Restriction; Loss of Surface Immunoglobulin in a B-Cell Population; Coexistence of Two Different Cell-Lineage Markers on the Same Cell Population; Expression of Immature Cell Markers in a Large Number of Cells; Selective Loss of One or More Cell-Lineage Antigens; Analysis of T-cell Receptor-Vâ (TCR-Vâ) Repertoire; Selection of Monoclonal Antibody Panels; Standard Panel; Two-Tiered Approach; Targeted Approach; Immunophenotyping of Acute Leukemia; Immunophenotyping of Lymphoma and Chronic Lymphoid Leukemia; Chronic Lymphocytic Leukemia/Small Lymphocytic Lymphoma; Mantel Cell Lymphoma; Prolymphocytic Leukemia; Follicular Lymphoma; Burkitt Lymphoma (BL) and Diffuse Large B-cell Lymphoma (DLBCL); Hairy Cell Leukemia; Marginal Zone B-Cell Lymphoma; Natural Killer Cell/Natural Killer-Like T-Cell Lymphoma; Peripheral T-Cell Lymphoma; Immunophenotyping of Plasmaproliferative Disorders; Detection of Minimal Residual Disease (MRD); Flow Cytometry Panels; Introduction; Chronic Lymphocytic Leukemia/Small Lymphocytic Lymphoma (CLL/SLL); Mantle Cell Lymphoma (MCL); Lymphoma of Follicular Cell Origin (FL); Hairy Cell Leukemia (HL); Plasma Cell Myeloma (PCM); Peripheral T-cell Lymphoma; Gamma-delta T-cell Lymphoma (G-D TCL); Mycosis Fungoides / Sezary Syndrome (MF/SS); Extranodal NK/T-cell Lymphoma, nasal type (NK/T L); Angioimmunoblastic T-cell Lymphoma (AITCL); Acute Lymphoblastic Leukemia (ALL); Acute Myeloid Leukemia (AML); Hematogones; Supplementary Techniques to Immunophenotyping; Cytochemical Staining; Immunohistochemistry; Molecular Biology; Cytogenetics; Lymphocyte Subset Analysis for Immunodeficiency States; Clinical Application; Technical Consideration; DNA Ploidy Analysis; The Cell Cycle; Ploidy; Major Parameters in DNA Analysis; DNA Index (DI); Coefficient of Variation (CV); G2M Peak versus Tetraploid Peak; Doublet Discrimination; S-Phase Fraction.; Clinical Application of DNA Analysis in Hematologic and Nonhematologic Neoplasms; Aneuploidy; S-phase Fraction; RNA Index; Reticulocyte Analysis; Clinical Application; Technical Consideration


Case 1; Case 2; Case 3; Case 4; Case 5; Case 6; Case 7; Case 8; Case 9; Case 10; Case 11; Case 12; Case 13; Case 14; Case 15; Case 16; Case 17; Case 18; Case 19; Case 20


Share this!