CACMLE Microbiology/Virology Courses

089.1-Online: Microbes That Make You Run! 2nd Edition

$100.00

Contact Hours (Points): 10

Infectious diarrheal disease is a major cause of morbidity and/or mortality in the world, resulting in many physician office visits and hospitalizations per year. Many of the agents involved are bacterial in origin, especially Gram negative rods belonging to the family Enterobacteriaceae. Some microbes that cause diarrheal disease may reside in the intestinal tract as normal flora, where they cause disease only under special circumstances. Other agents are not a part of the normal intestinal flora but rather cause infection and invade via exogenous exposure by ingestion of contaminated food or water, or by direct contact with an infected individual. This course examines common bacterial stool pathogens involved in gastrointestinal disease and diarrhea, including the genera Salmonella, Shigella, Campylobacter, and several pathogenic serotypes of Escherichia coli. The course includes a discussion of the potential sources of infection; the typical clinical presentations associated with these pathogens as well as a brief discussion of pathogenic mechanisms. Isolation, identification and characterization of the bacteria both by traditional and newer methodologies will be discussed. Treatment, along with efficacy of antibiotic susceptibility testing, will be considered.

Course emphasis will primarily be placed on routine stool culture procedures including isolation of the Enterobacteriaceae and Campylobacter. However, there are several other bacterial organisms implicated in gastrointestinal disease. A brief review and discussion of Yersinia enterocolitica, Vibrio cholerae, Vibrio parahemalyticus, Aeromonas hydrophila, Plesiomonas shigelloides, and Clostridium difficile diarrheal infections, along with their laboratory isolation procedures, will also be included.

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Product Description

Microbes That Make You Run! 2nd Edition

Self-Study 089.1 – Basic Level

Contact Hours (Points): 10

Major Revisions by Jeanne Stoddard, MHS, MT(ASCP). Originally written by Patricia A. Collins, MS, MT(ASCP)SM.

Published 2001. Revised 2014.

Online – 089.1: Course Taken Online: $100.00 BUY NOW

IMPORTANT: This online course is accessible using the following:

Desktop or Laptop Computer – Download the latest Adobe Reader program.

Kindle Fire – No additional App download is necessary.

Apple iPad (all versions) / Nook / Kindle – Recommend purchase of a PDF Reader App, such as GoodReader, Cloudreaders, or Stanza by Amazon. Once downloaded, launch this App to access the course online site in order to logon to and view your course.

TABLE OF CONTENTS

INTRODUCTION

Molecular Methods: PCR Amplification; Pulse-Field Gel Electrophoresis (PFGE); Mass Spectrometry – MALDI-TOF

SECTION I: ROUTINE STOOL CULTURE PROCEDURES

Routine and Selective Culture Media; Enrichment Broths; Specialized Culture Media; Serologic Testing; Fecal Gram Stains and Fecal Lactoferrin

SECTION II: ESCHERICHIA COLI

Clinical Infection; Pathogenicity; Enteropathogenic Escherichia coli (EPEC), Enteroadherent Eschericia coli (EAEC); Clinical Syndromes; Organism Identification and Treatment; Enterotoxigenic Escherichia coli (ETEC); Clinical Infection; Organism Identification; Vaccines; Treatment;

Enterohemorrhagic Escherichia coli (EHEC), Shiga-toxin Producing Escherichia coli (STEC); E. coli O157:H7 Infection History and Nomenclature; Shiga-toxin Producing Escherichia coli (STEC) Associated Infection Epidemiology; Pathogenicity; Transmission; Clinical Infection; Organism Identification: Culture and Non-Clture Methods; Growth on Selective and Non-Selective Media; Biochemical Reactions; Shiga Toxin Detection; Non-culture Assays; Treatment; Enteroinvasive Escherichia coli (EIEC); Clinical Infection; Pathogenicity and Transmission; Organism Identification; Treatment; Enteroaggregative Escherichia coli (EAEC); Clinical Infection; Epidemiology and Pathogenicity; Organism Identification; Microarray Diagnostic Methods; Treatment

SECTION III: SALMONELLA SPECIES

Classification and Nomenclature History; Current Classification and Nomenclature; Clinical Infection Overview ; Epidemiology and Transmission; Salmonella Pathogenicity; Immune Response; Vi Antigen; Clinical Infections; Enteric (Typhoid) Fever; Treatment; Vaccines; Organism Identification; Growth on Routine Culture Media; Biochemical Reactions; Molecular Methods; Antimicrobial Resistance

SECTION IV: SHIGELLA SPECIES

Transmission; Epidemiology and Historical Perspective; Clinical Symptoms; Pathogenicity and Virulence; Organism Identification; Growth on Routine Culture Media; Biochemical Reactions; Treatment; Vaccines; Identification of Outbreaks

SECTION V: CAMPYLOBACTER JEJUNI

Transmission; Clinical Symptoms; Organism Isolation; Organism identification; Campylobacter Selective Media; Presumptive Identification; Antigen Testing; Treatment

SECTIONI VI: YERSINIA SPECIES

Yersinia enterocolitica and Yersinia pseudotuberculosis; Transmission; Clinical Infection and Symptoms; Epidemiology; Clinical Infection with Yersinia pestis (Plague); Plague Treatment; Gastroenteritis Treatment; Organism Identification; Growth on Routine Culture Media; Biochemical Reactions; Molecular Methods

SECTION VII: VIBRIO CHOLERAE, VIBRIO PARAHEMOLYTICUS, AEROMONAS, AND PLESIOMONAS

Vibrio cholerae; Clinical Symptoms; Treatment; Vibrio parahemolyticus; Clinical Symptoms; Organism Identification; Culture Media; Biochemical Reactions; Aeromonas species; Clinical Symptoms; Treatment; Organism Identification; Culture Media; Biochemical Reactions; Plesiomonas shigelloides; Clinical Symptoms; Treatment; Organism Identification; Culture Media; Biochemical Reactions

SECTION VIII: CLOSTRIDIUM DIFFICILE

Epidemiology and Transmission; Clinical Symptoms; Treatmen; Organism Identification; Growth on Selective Culture Media; Molecular and Antigen Detection Methods

CONCLUSION

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