Anaerobic Bacteriology for Today’s Clinical Laboratory
Self-Study 117.2 – Basic Level
Contact Hours (Points): 12
Revised by Robert C. Fader, PhD, D(ABMM) in 2010; Updated in 2014.
Originally written by Paul G. Engelkirk PhD, MT(ASCP) and Janet Duben-Engelkirk EdD, MT(ASCP) in 2004.
Online – 117.2: Course Taken Online: $120.00 BUY NOW
IMPORTANT: This online course is accessible using the following:
Desktop or Laptop Computer – Download the latest Adobe Reader program.
Kindle Fire – No additional App download is necessary.
Apple iPad (all versions) / Nook / Kindle – Recommend purchase of a PDF Reader App, such as GoodReader, Cloudreaders, or Stanza by Amazon. Once downloaded, launch this App to access the course online site in order to logon to and view your course.
Here’s what satisfied customers have to say~
“This course was extremely educational and contributed a great deal to my success as a microbiologist.”
“Quite enjoyed this course.“
TABLE OF CONTENTS
SECTION I: INTRODUCTION TO ANAEROBES AND CLINICAL ANAEROBIC BACTERIOLOGY
What are Anaerobes?, Classifying Bacteria Based on Their Atmospheric Requirements, Why is Oxygen Lethal to Anaerobes?, The First Anaerobes, Who Discovered Anaerobes?, Where Are Anaerobes Found?, The Importance of Anaerobes, Debunking the Myths about Clinical Anaerobic Bacteriology, Furthering Your Knowledge of Clinical Anaerobic Bacteriology
SECTION II: INTRODUCTION TO CLINICALLY SIGNIFICANT ANAEROBES AND THE DISEASES THEY CAUSE
Taxonomic Classification of Clinically Encountered Anaerobes, Spore-forming, Anaerobic Gram-Positive Bacilli (Clostridium spp), Nonsporeforming, Anaerobic, Gram-Positive Bacilli, Non-spore-forming, Anaerobic, Gram-Negative Bacilli, Other Anaerobic, Gram-Negative Bacilli, Anaerobic, Gram-Negative Cocci, Anaerobic, Gram-Positive Cocci, A Word About Taxonomy Changes, Anaerobes of the Indigenous Microflora (Endogenous Anaerobes), Anaerobes Associated with Specific Anatomical Sites, Upper Respiratory Tract, Skin, Urethra, Vagina, Colon, The Role of Anaerobes in Human Diseases, Diseases Caused by Anaerobes of Exogenous Origin, Diseases Caused by Anaerobes of Endogenous Origin, Virulence Factors of Anaerobic Bacteria, Indications of Anaerobe Involvement in Human Disease, Anaerobes Isolated Most Frequently from Clinical Specimens
SECTION III: SELECTING, COLLECTING, TRANSPORTING, AND PROCESSING CLINICAL SPECIMENS FOR ANAEROBIC BACTERIOLOGY
Selection of Clinical Specimens for Anaerobic Bacteriology, Acceptable Specimens, Unacceptable Specimens, Collection and Transport of Specimens for Anaerobic Bacteriology, Aspirates, Swabs, Tissue, Blood, Processing of Specimens for Anaerobic Bacteriology, Macroscopic Examination of Specimens, Microscopic Examination of Specimens, Specimen Processing Techniques, Aspirates, Tissue and Bone Fragments, Swabs, Inoculation of Appropriate, Plated and Tubed Media, Culture Media Recommendations, Media for Use in the Primary Isolation Setup, Plates to be Incubated Aerobically, Anaerobic Incubation of Inoculated Media, Anaerobic Chambers, Anaerobic Jars, Anaerobic Bags and Pouches, Evacuation-Replacement System, Common Errors Pertaining to Specimen Collection and Processing
SECTION IV: METHODS FOR IDENTIFYING (SPECIATING) CLINICALLY SIGNIFICANT ANAEROBES
Processing of Isolates Suspected of Being Anaerobes, Examination of Primary Plates, When Should Primary Plates Be Examined?, Indications of the Presence of Anaerobes, Examination of Specific Plates Included in the Primary Isolation Setup, Processing of Colonies Suspected of Being Anaerobes, Colony Morphology, Gram Reaction and Cellular Morphology, Inoculation of a Pure Culture/Subculture (PC/SC) Plate, Disks to Add to the PC/SC Plate, Aerotolerance Testing, Review of Processing Procedures and Rationale for Identification of Anaerobic Isolates, Presumptive Identification of Anaerobes, Presumptive Identification of Gram negative Anaerobes by CLSI M-35A, Presumptive Identification of Gram positive Anaerobes by CLSI M-35A, Further Inexpensive Tests Used to Presumptively Identify Anaerobic Isolates, Logical, Stepwise Approach to Presumptive Identification of Anaerobic Isolates, PRES-ID Section 1: Disks to Add to the Pure Culture/Subculture Plate, PRES-ID Section 2: Presumptive Identification of Anaerobic Gram-Positive Cocci, PRES-ID Section 3: Presumptive Identification of Anaerobic Gram-Negative Cocci, PRES-ID Section 4: Presumptive Identification of Sporeforming Gram-Positive or Gram-Negative Bacilli and Coccobacilli, PRES-ID Section 5: Presumptive Identification of Nonsporeforming Gram-Positive Bacilli, (NSFGPB) and Coccobacilli, PRES-ID Section 6: Interpretation of Special Potency Antimicrobial Disk Results, PRES-ID Section 7: Presumptive Identification of Porphyromonas Species, PRES-ID Section 8: Presumptive Identification of Members of the Bacteroides fragilis Group, PRES-ID Section 9: Presumptive Identification of Prevotella Species, PRES-ID Section 10: Presumptive Identification of Other Anaerobic Gram-Negative Bacilli (GNB) and Coccobacilli, Definitive Identification of Anaerobes – When and How?, “Conventional” Tubed Biochemical Identification Systems, Biochemical-Based Minisystems for Anaerobe Identification, Pre-existing Enzyme-Based Minisystems for Anaerobe Identification, Use of Tablets to Identify Anaerobes, Identification of Anaerobes via Metabolic End-Product Analysis by Gas-Liquid Chromatography (GLC), Cellular Fatty Acid Analysis by High-Resolution GLC, The MIDI Sherlock System,
16s rRNA gene sequencing, Common Errors Regarding Identification (Speciation)
SECTION V: SUSCEPTIBILITY TESTING OF CLINICALLY SIGNIFICANT ANAEROBES
When Should Susceptibility Testing of Anaerobes be Performed?, Specific Antimicrobial Agents to be Tested, Susceptibility Testing Options, E-test®, Quality Control, Additional Comments Regarding Susceptibility Testing of Anaerobes, Beta-Lactamase Testing, Treating Anaerobe-Associated Infectious Processes/Diseases, Antimicrobial Agents Active against Anaerobes