CACMLE Immunology/Serology/Molecular Courses

133.1-Online: Understanding Molecular Pathology – Methods and Applications, 2nd Edition


Contact Hours (Points): 10

Molecular diagnostics has established itself as a mainstay of laboratory medicine and a foundation for personalized and predictive medicine. This new revised edition continues to emphasize the fundamental principles needed by the newcomer preparing for bench training and actual clinical laboratory test performance. Though not an exhaustive account, this self-study can also serve as the foundation for preparation for certification exams in molecular diagnostics.

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Product Description

Understanding Molecular Pathology: Methods and Applications, 2nd Edition

Self-Study 133.1 – Basic to Intermediate Level

Contact Hours (Points): 10

Written by Gregory L. Blakey MD and Daniel Farkas PhD, HCLD, CC, CLSp(MB)

Published 2006. Revised in 2014 by Lindsy Hengesbach, MS, MB(ASCP)CM.

In describing current molecular diagnostics practice, a certain amount of references to for-profit companies was unavoidable. We have endeavored to be balanced and unbiased in these matters; no endorsements are intended.

Online – 133.1: Course Taken Online: $100.00 BUY NOW

IMPORTANT: This online course is accessible using the following:

Desktop or Laptop Computer – Download the latest Adobe Reader program.

Kindle Fire – No additional App download is necessary.

Apple iPad (all versions) / Nook / Kindle – Recommend purchase of a PDF Reader App, such as GoodReader, Cloudreaders, or Stanza by Amazon. Once downloaded, launch this App to access the course online site in order to logon to and view your course.


SECTION 1: MILESTONES IN MOLECULAR BIOLOGY AND BASIC NUCLEIC ACID BIOCHEMISTRY: Biochemistry; Complementarity; DNA Replication; Transcription and Translation

SECTION 2: SPECIMEN COLLECTION, NUCLEIC ACID ISOLATION, AND STORAGE; Nucleic Acid Purification; Nucleic Acid Quantification;

Storage requirements for purified DNA; Storage requirements for purified RNA; Consequences of DNA Degradation

SECTION 3: DNA HYBRIDIZATION: Restriction Endonuclease Digestion of DNA; Blotting; Pre-hybridization; Hybridization; Blot Washing and Development; Stringency

SECTION 4: NUCLEIC ACID AMPLIFICATION: PCR Basics; Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR); Touch Down PCR; Hot Start PCR; Multiplex PCR; Long PCR; Sequence Specific Primer and Amplification Refractory Mutation System; Real-time PCR; PCR Inhibitors;

PCR Alternatives; Amplicon Carryover Contamination


Sequencing Methodology; PyroSequencing; Next Generation Sequencing; Fragment Analysis

SECTION 6: CANCER DIAGNOSTICS: Hematopoietic Malignancies; Gene rearrangement studies; bcr-abl testing; t(14;18) testing; Solid tumors; HER-2/neu testing; Tumor suppressor gene testing; Screening tests; Geretic Targets; Epigenetic Changes and Next Generation Sequencing in Cancer Diagnostics

SECTION 7: INFECTIOUS DISEASES TESTING: Molecular Virology; Molecular Bacteriology

SECTION 8: GENETIC TESTING: Cystic Fibrosis (CF); Fragile X Syndrome; Hereditary Hypercoagulability; Hereditary Hemochromatosis; Histocompatibility; Biospecimen Banking; Ethics

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