Methods of Identification and Susceptibility Testing for Clinically Significant Anaerobes – Section 3 of “Anaerobic Bacteriology for Today’s Clinical Laboratory”
Self-Study 167 – Basic Level
Contact Hours (Points): 6
Revised by Robert C. Fader, PhD, D(ABMM) in 2010; Updated in 2014.
Originally written by Paul G. Engelkirk PhD, MT(ASCP) and Janet Duben-Engelkirk EdD, MT(ASCP) in 2004.
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TABLE OF CONTENTS
SECTION IV: METHODS FOR IDENTIFYING (SPECIATING) CLINICALLY SIGNIFICANT ANAEROBES
Processing of Isolates Suspected of Being Anaerobes, Examination of Primary Plates, When Should Primary Plates Be Examined?, Indications of the Presence of Anaerobes, Examination of Specific Plates Included in the Primary Isolation Setup, Processing of Colonies Suspected of Being Anaerobes, Colony Morphology, Gram Reaction and Cellular Morphology, Inoculation of a Pure Culture/Subculture (PC/SC) Plate, Disks to Add to the PC/SC Plate, Aerotolerance Testing, Review of Processing Procedures and Rationale for Identification of Anaerobic Isolates, How far do we need to go toidentify the isolate? Presumptive Identification of Anaerobes, Presumptive Identification of Gram negative Anaerobes by CLSI M-35A, Presumptive Identification of Gram positive Anaerobes by CLSI M-35A, Further Inexpensive Tests Used to Presumptively Identify Anaerobic Isolates, Logical, Stepwise Approach to Presumptive Identification of Anaerobic Isolates, PRES-ID Section 1: Disks to Add to the Pure Culture/Subculture Plate, PRES-ID Section 2: Presumptive Identification of Anaerobic Gram-Positive Cocci, PRES-ID Section 3: Presumptive Identification of Anaerobic Gram-Negative Cocci, PRES-ID Section 4: Presumptive Identification of Sporeforming Gram-Positive or Gram-Negative Bacilli and Coccobacilli, PRES-ID Section 5: Presumptive Identification of Nonsporeforming Gram-Positive Bacilli, (NSFGPB) and Coccobacilli, PRES-ID Section 6: Interpretation of Special Potency Antimicrobial Disk Results, PRES-ID Section 7: Presumptive Identification of Porphyromonas Species, PRES-ID Section 8: Presumptive Identification of Members of the Bacteroides fragilis Group, PRES-ID Section 9: Presumptive Identification of Prevotella Species, PRES-ID Section 10: Presumptive Identification of Other Anaerobic Gram-Negative Bacilli (GNB) and Coccobacilli, Definitive Identification of Anaerobes – When and How?, “Conventional” Tubed Biochemical Identification Systems, Biochemical-Based Minisystems for Anaerobe Identification, Pre-existing Enzyme-Based Minisystems for Anaerobe Identification, Use of Tablets to Identify Anaerobes, Cellular Fatty Acid Analysis by High-Resolution GLC, MIDI Sherlock Microbial Identification System, 16s rRNA gene sequencing, Mass Spectrometry, Common Errors Regarding Identification (Speciation)
SECTION V: SUSCEPTIBILITY TESTING OF CLINICALLY SIGNIFICANT ANAEROBES
When Should Susceptibility Testing of Anaerobes be Performed?, Specific Antimicrobial Agents to be Tested, Susceptibility Testing Options, E-test®,
Quality Control, Additional Comments Regarding Susceptibility Testing of Anaerobes, Beta-Lactamase Testing, Treating Anaerobe-Associated Infectious Processes/Diseases, Antimicrobial Agents Active against Anaerobes