Understanding Molecular Pathology: Background and Methods – Section 1 of “Understanding Molecular Pathology: Methods and Applications, 2nd Edition”
Self-Study 248 – Basic to Intermediate Level
Contact Hours (Points): 8
Written by Gregory L. Blakey MD and Daniel Farkas PhD, HCLD, CC, CLSp(MB)
Published 2006. Revised in 2014 by Lindsy Hengesbach, MS, MB(ASCP)CM
In describing current molecular diagnostics practice, a certain amount of references to for-profit companies was unavoidable. We have endeavored to be balanced and unbiased in these matters; no endorsements are intended.
Online – 248: Course Taken Online: $96.00 BUY NOW
IMPORTANT: This online course is accessible using the following:
Desktop or Laptop Computer – Download the latest Adobe Reader program.
Kindle Fire – No additional App download is necessary.
Apple iPad (all versions) / Nook / Kindle – Recommend purchase of a PDF Reader App, such as GoodReader, Cloudreaders, or Stanza by Amazon. Once downloaded, launch this App to access the course online site in order to logon to and view your course.
TABLE OF CONTENTS
SECTION 1: MILESTONES IN MOLECULAR BIOLOGY AND BASIC NUCLEIC: ACID BIOCHEMISTRY; Biochemistry; Complementarity; DNA Replication; Transcription and Translation
SECTION 2: SPECIMEN COLLECTION, NUCLEIC ACID ISOLATION, AND STORAGE: Nucleic Acid Purification; Nucleic Acid Quantification; Storage Requirements for Purified DNA; Storage Requirements for Purified RNA; Consequences of DNA Degradation
SECTION 3: DNA HYBRIDIZATION: Restriction Endonuclease Digestion of DNA; Blotting; Pre-hybridization; Hybridization; Blot Washing and Development; Stringency
SECTION 4: NUCLEIC ACID AMPLIFICATION: PCR Basics; Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR); Touch Down PCR; Hot Start PCR; Multiplex PCR; Long PCR; Sequence Specific Primer and Amplification Refractory Mutation System; Real-time PCR; PCR Inhibitors; PCR Alternatives; Amplicon Carryover Contamination
SECTION 5: DNA SEQUENCING AND FRAGMENT ANALYSIS: Sequencing Methodology; PyroSequencing; Next Generation Sequencing; Fragment Analysis