CACMLE Microbiology/Virology Courses

275-Online: User-Friendly Techniques to Identify Resistance Mechanisms in Gram-Negative Bacilli

$60.00

Contact Hours (Points): 5

This course consists of 4 sessions. Each session focuses on the origin and action of specific resistance mechanisms, and discusses the procedures to optimize detection, as well as appropriate reporting of this resistance in order to help guide patient care.

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Product Description

User-Friendly Techniques to Identify Resistance Mechanisms in Gram-Negative Bacilli

Self-Study 275 – Intermediate Level

Contact Hours (Points): 5

Written by Paul C. Schreckenberger, Ph.D.

Published 2014, Reformatted 2015.
Included are:

  • Procedure for the 12-Disk Method
  • Procedures for the Hodge Test and MBL Etest
  • Tools for interpreting AST results
  • Appropriate post-analytic comments to add to AST reports

Session #1: Extended Spectrum Beta-lactamases (ESBLs); K1 Beta-lactamase; Introduction to the 12-Disk Method

At the completion of this Session, the participant will be able to:

  • Recognize ESBL and K1 Beta-lactamase resistance mechanisms by observing patterns of susceptibility with the 12 disk test method.
  • Describe the procedure to set up and read the 12 disk test plate.
  • Modify antibiotic interpretations, based on the resistance mechanism.
  • Add appropriate interpretive comments to susceptibility reports to aid in physician understanding.

Session #2: AmpC Beta-lactamases; Organisms Possessing both AmpC and ESBLs

At the completion of this Session, the participant will be able to:

  • Identify both ESBL and AmpC Beta-lactamase resistance mechanisms by observing patterns of susceptibility on the 12-disk test plate.
  • List the organisms commonly associated with these resistance mechanisms.
  • Modify antibiotic interpretations based on identified resistance mechanisms.
  • List the Classes and describe the mode of action of AmpC and ESBLs.

Session #3: Carbapenem Resistant Enterobacteriaceae (CRE); Klebsiella pneumoniae Carbapenemase (KPC); New Delhi Metallo – beta-lactamase (NDM); SME, NMCA, & Oxa-type carbapenemases

At the completion of this Session, the participant will be able to:

  • Identify the best methods for detecting metallo-beta-lactamase resistance.
  • List the pitfalls of susceptibility testing for the detection of carbapenemases.
  • Describe when to add interpretive comments to susceptibility reports to aid in the understanding of the importance of these mechanisms as they relate to patient care and infection comtrol.
  • Describe the procedure, interpretation and application of the Hodge Test and MBL Etest.

The Homework Assignment following this Session.

The assignment applies (via case studies, digital images and AST results) the resistance mechanisms discussed in Sessions #1 – #3.

Session #4: Appropriate AST Result Reporting; Procedures to Detect & Resolve Misidentified or Unexpected Results (from Homework Assignment)

At the conclusion of the discussion for the Homework Assignment, the participant will be able to:

  • Evaluate AST results
  • Describe additional testing to identify the resistance mechanism, if appropriate
  • Identify the specific resistance mechanism(s)
  • Provide relevant antimicrobial test reporting
  • List appropriate post-analytic comments
  • Resolve misidentified and unexpected results

Presented in an easy-to-use PowerPoint slide format with corresponding audio, it’s just like being at a live presentation!

Online 275: Course Taken Online: $60.00 BUY NOW

IMPORTANT: This online course is NOT downloadable; and is accessible using the following:

Desktop or Laptop Computer – Download the Adobe Flash program.

Apple iPad – Purchase for download Photon Flash Player for iPad Flash App or iSwifter Flash App. Launch either of these Apps to access the course online site in order to logon to and view your course. Remember to adjust your audio.

~~~ Sorry, you cannot view this course on a Nook or Kindle. ~~~

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