CACMLE Hematology Courses

276-Online: Principles of Flow Cytometry – Section 1 of “Principles and Clinical Applications of Flow Cytometry, 4th Edition”

$48.00

Contact Hours (Points): 4

This revised course reviews the technical principles of flow cytometry, including the structure and mechanism of instrumentation and quality control. After completing the course, participants should be able to understand how the instrument works and how to select a monoclonal antibody panel.This course was revised in 2014.

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Product Description

Principles of Flow Cytometry – Section 1 of “Principles and Clinical Applications of Flow Cytometry, 4th Edition”

Self-Study 276 – Intermediate to Advanced Level

Contact Hours (Points): 4

Written by Tsieh Sun MD in 2003.

Revised in 2014 by Susan A. McQuiston, JD, MLS(ASCP).

Online – 276: Course Taken Online: $48.00 BUY NOW

IMPORTANT: This online course is accessible using the following:

Desktop or Laptop Computer – Download the latest Adobe Reader program.

Kindle Fire – No additional App download is necessary.

Apple iPad (all versions) / Nook / Kindle – Recommend purchase of a PDF Reader App, such as GoodReader, Cloudreaders, or Stanza by Amazon. Once downloaded, launch this App to access the course online site in order to logon to and view your course.

Here’s what satisfied customers have to say~

“Great course.”
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TABLE OF CONTENTS
SECTION 1: INTRODUCTION
SECTION 2: INSTRUMENTATION
Fluid Transport System; Optical System; Fluorescent Signals; Lens, Filter, and Mirror; Electronic System; Computer System; Scattergram (dot plot, cytogram); Single Histogram; Contourgram (contour plot, contour map, contour histogram); Isometric Plot (three-dimensional isometric curve); Cell Sorter
SECTION 3: QUALITY ASSURANCE
Factors Affecting Flow Cytometry Results; Specimen Problems; Reagent Problems; Parameters for Cell Distinction; Cell Size; Cytoplasmic Granularity; Immunophenotyping; Comparison of Percentages of Reactive Cells; The Intensity of Immunofluorescence of Surface Immunoglobulin; Double-labeling; DNA and RNA Contents; Cells That Are Not Distinguishable; Designing an 8-10 Color Assay; Current FDA Requirements Related to Flow Cytometry Antibody Conjugate Cocktails
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