Principles of Flow Cytometry – Section 1 of “Principles and Clinical Applications of Flow Cytometry, 4th Edition”
Self-Study 276 – Intermediate to Advanced Level
Contact Hours (Points): 4
Written by Tsieh Sun MD in 2003.
Revised in 2014 by Susan A. McQuiston, JD, MLS(ASCP).
Online – 276: Course Taken Online: $48.00 BUY NOW
IMPORTANT: This online course is accessible using the following:
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TABLE OF CONTENTS
SECTION 1: INTRODUCTION
SECTION 2: INSTRUMENTATION
Fluid Transport System; Optical System; Fluorescent Signals; Lens, Filter, and Mirror; Electronic System; Computer System; Scattergram (dot plot, cytogram); Single Histogram; Contourgram (contour plot, contour map, contour histogram); Isometric Plot (three-dimensional isometric curve); Cell Sorter
SECTION 3: QUALITY ASSURANCE
Factors Affecting Flow Cytometry Results; Specimen Problems; Reagent Problems; Parameters for Cell Distinction; Cell Size; Cytoplasmic Granularity; Immunophenotyping; Comparison of Percentages of Reactive Cells; The Intensity of Immunofluorescence of Surface Immunoglobulin; Double-labeling; DNA and RNA Contents; Cells That Are Not Distinguishable; Designing an 8-10 Color Assay; Current FDA Requirements Related to Flow Cytometry Antibody Conjugate Cocktails